User can search specific ncRNA or pathway by its id or name in ANNOTATION. While in ENRICHMENT, user can obtain the integrated result of certain ncRNAs or pathways, to find out enriched pathways or ncRNAs. Also, user can directly download annotation results of ncFN.

1. To annotate a ncRNA, user need to first select one ncRNA type from the dropdown list. Then, input the ncRNA ID. It is worth noting that miRNA IDs are started with "hsa-let" or "hsa-miR", lncRNA IDs are gene symbols, and circRNA IDs are collected from circBase, starting with "hsa_circ". User can select different ncRNA type to see the example ID. Finally, the P-value threshold of annotation result can be defined by user (Fig 1). 2. To annotate a pathway, user need to select one pathway from the dropdown list, including 299 KEGG pathways. User can filter annotation result by inputting the P-value threshold (Fig 2).

1. To perform enrichment analysis for multiple ncRNAs, user need to first select one ncRNA type or "All" from the dropdown list, depending on the input ncRNA IDs. Then, input the ncRNA ID. It is worth noting that miRNA IDs are started with "hsa-let" or "hsa-miR", lncRNA IDs are gene symbols, and circRNA IDs are collected from circBase, starting with "hsa_circ". Finally, input the P-value threshold of enrichment analysis (Fig 3). 2. To perform enrichment analysis for multiple pathways, user need to select several pathways from the list, including 299 KEGG pathways. User can filter enrichment result by inputting the P-value threshold (Fig 4).